Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging
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چکیده
منابع مشابه
Enhanced Beetle Luciferase for High-Resolution Bioluminescence Imaging
We developed an enhanced green-emitting luciferase (ELuc) to be used as a bioluminescence imaging (BLI) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase (FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visu...
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UNIVERSITY OF EASTERN FINLAND, Faculty of Science and Forestry Programme of biosciences, major: biochemistry Teemu Rönkkö: Novel Luciferase Inhibitors and Peptide Conjugates for Bioluminescence Imaging Master of Science thesis 66 pages Supervisors: PhD Juha Pulkkinen and MSc Pekka Poutiainen February 2013 ___________________________________________________________________________
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Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that is widely used as a model organism for the analysis of infection biology. In this context, there is a current need to develop improved reporters for enhanced bioluminescence imaging (BLI) of the pathogen in infection models. We have developed a click beetle red luciferase (CBR-luc) based vector (pPL2CBRopt) expres...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2010
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0010011